mammocult basal medium with proliferation supplement  (STEMCELL Technologies Inc)

Journal: Cell reports

Article Title: Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer

doi: 10.1016/j.celrep.2019.05.084

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mammospheres were cultured in MammoCult Human Basal Medium with added Proliferation Supplement (StemCell Technologies, #05621 & #05622) on Costar Ultra Low Attachment tissue culture plates (Corning, #3471).

Techniques: Virus, Plasmid Preparation, Recombinant, Invasion Assay, Migration, Purification, Sequencing, Gene Expression, shRNA, Software

Journal: Cell reports

Article Title: Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer

doi: 10.1016/j.celrep.2019.05.084

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MammoCult Human Basal Medium plus proliferation supplement , StemCell Technologies , Cat# 05621 and # 05622.

Techniques: Virus, Plasmid Preparation, Recombinant, Invasion Assay, Migration, Purification, Sequencing, Gene Expression, shRNA, Software

Journal: British Journal of Cancer

Article Title: Silencing NOTCH signaling causes growth arrest in both breast cancer stem cells and breast cancer cells

doi: 10.1038/bjc.2013.642

Figure Lengend Snippet: Breast cancer stem cells exhibit aggressive growth and resistance to chemotherapeutic agents: ( A ) Aldefluor assay showing the percentage of ALDH − and ALDH + cells population in MDA-MB-231 cells by flow cytometry analysis. ( B ) To check cell proliferation, ALDH − and ALDH + 25 × 10 3 cells were plated in 24-well plates and allowed to grow for 24 h followed by harvesting and counting of viable cells using a trypan blue exclusion assay. ( C ) To assess anchorage-independent growth, 5 × 10 3 cells (ALDH − and ALDH + cells) were grown in soft agar for 10 days. Colonies were stained with crystal violet and counted manually. ( D ) A mammosphere assay was performed using 4 × 10 3 cells 2 ml −1 of DMEM/Mammocult media in ultra-low attachment plates. The cells were allowed to grow for 2 weeks, and then mammospheres were counted. ( E ) ALDH − , ALDH + , and commercial BCSCs were plated in six-well plates and grown until confluent. A uniform wound was created in the center of the monolayer. The wound gap was photographed in a Biostation CT programmed to take pictures every 2 h. The distance between the edges of wound was measured in μ M using NIS-Element software, and statistical analysis was performed. ( F ) A transwell invasion assay was performed with ALDH − cells, ALDH + cells, and commercial BCSCs using Boyden chambers. The invasive cells were stained with crystal violet and counted. Data are expressed as mean±s.e.m. of two independent experiments. Student's t -test was used to calculate statistical significance. * P <0.05, ** P <0.005, and *** P <0.0001.

Article Snippet: The same number of cells was cultured in MammoCult basal medium containing MammoCult proliferation supplements, 4 μ g ml −1 of heparin, and 0.48 μ g ml −1 of hydrocortisone (StemCell Technologies) in ultra-low attachment plates (Corning, Acton, MA, USA).

Techniques: Flow Cytometry, Trypan Blue Exclusion Assay, Staining, Software, Transwell Invasion Assay